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Antibodies bound to the blocking peptide no more bind to the epitope around the focus on protein. By evaluating the staining within the blocked antibody versus the antibody by itself, you can see which staining is distinct.
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This guide outlines even further feasible optimization of mobile and immunoassay parameters to ensure the absolute best success are...
This guidebook outlines even further feasible optimization of cellular and immunoassay parameters to be certain the best possible benefits are...
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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Blocking peptides are peptides that bind specifically towards the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which contribute to your temporal coordination of every mitotic event. Cyclin E sorts a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. check here This protein accumulates with the G1-S stage boundary and is particularly degraded as cells development via S section.
Overexpression of this gene continues to be noticed in several tumors, which leads to chromosome instability, and so may possibly contribute to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital role in selling mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two more splice variants were described but detailed nucleotide sequence details is not really however readily available.
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